Development of engineered endolysins with in vitro intracellular activity against streptococcal bovine mastitis-causing pathogens.

ABSTRACT: Bacteriophage-derived endolysins are a novel class of antimicrobials known to rapidly kill bacteria, including antibiotic-resistant strains. We here engineered endolysins against the bovine mastitis pathogens Streptococcus uberis, Streptococcus agalactiae and Streptococcus dysgalactiae, also targeting intracellular survival and biofilm formation. For this purpose, high-throughput DNA assembly was used to create a library with >80,000 theoretical endolysin variants for screening of their bacteriolytic activity against Gram-positive isolates from (sub)clinically affected cows. This lytic activity was evaluated by turbidity reduction and time-kill assays in phosphate-buffered saline and pasteurized whole cow's milk to allow a rank up of the most potent leading candidates. A top candidate was

SUBMITTER: Vander Elst N 

PROVIDER: S-EPMC10686134 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature