Anionic omega currents from single countercharge mutants in the voltage-sensing domain of Ci-VSP.

ABSTRACT: The S4 segment of voltage-sensing domains (VSDs) directly responds to voltage changes by reorienting within the electric field as a permion. A narrow hydrophobic "gasket" or charge transfer center at the core of most VSDs focuses the electric field into a narrow region and catalyzes the sequential and reversible translocation of S4 positive gating charge residues across the electric field while preventing the permeation of physiological ions. Mutating specific S4 gating charges can cause ionic leak currents through the VSDs. These gating pores or omega currents play important pathophysiological roles in many diseases of excitability. Here, we show that mutating D129, a key countercharge residue in the Ciona intestinalis voltage-sensing phosphatase (Ci-VSP), leads to the generation of uniqu

SUBMITTER: Shen R 

PROVIDER: S-EPMC10686229 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature