Drug resistance and vaccine target surveillance of Plasmodium falciparum using nanopore sequencing in Ghana.

ABSTRACT: Malaria results in over 600,000 deaths annually, with the highest burden of deaths in young children living in sub-Saharan Africa. Molecular surveillance can provide important information for malaria control policies, including detection of antimalarial drug resistance. However, genome sequencing capacity in malaria-endemic countries is limited. We designed and implemented an end-to-end workflow to detect Plasmodium falciparum antimalarial resistance markers and diversity in the vaccine target circumsporozoite protein (csp) using nanopore sequencing in Ghana. We analysed 196 clinical samples and showed that our method is rapid, robust, accurate and straightforward to implement. Importantly, our method could be applied to dried blood spot samples, which are readily collected in endemic sett

SUBMITTER: Girgis ST 

PROVIDER: S-EPMC10686832 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature