Antibody fragments functionalized with non-canonical amino acids preserving structure and functionality - A door opener for new biological and therapeutic applications.

ABSTRACT: Functionalization of proteins by incorporating reactive non-canonical amino acids (ncAAs) has been widely applied for numerous biological and therapeutic applications. The requirement not to lose the intrinsic properties of these proteins is often underestimated and not considered. Main purpose of this study was to answer the question whether functionalization via residue-specific incorporation of the ncAA N⁶-[(2-Azidoethoxy) carbonyl]-l-lysine (Azk) influences the properties of the anti-tumor-necrosis-factor-α-Fab (FTN2). Therefore, FTN2_Azk variants with different Azk incorporation sites were designed and amber codon suppression was used for production. The functionalized FTN2_Azk variants were efficiently produced in fed-batch like μ-bioreactor cultivation

SUBMITTER: Hanaee-Ahvaz H 

PROVIDER: S-EPMC10686840 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature