Immunoinformatics analysis of Brucella melitensis to approach a suitable vaccine against brucellosis.

ABSTRACT:

Background

Brucellosis caused by B. melitensis is one of the most important common diseases between humans and livestock. Currently, live attenuated vaccines are used for this disease, which causes many problems, and unfortunately, there is no effective vaccine for human brucellosis. The aim of our research was to design a recombinant vaccine containing potential immunogenic epitopes against B. melitensis.

Methods

In this study, using immunoinformatics approaches, 3 antigens Omp31, Omp25, and Omp28 were identified and the amino acid sequence of the selected antigens was determined in NCBI. Signal peptides were predicted by SignaIP-5.0 server. To predict B-cell epitopes from ABCpred and Bcepred servers, to predict MHC-I epitopes from RANKPEP and SYFPEITHI servers, to predict

SUBMITTER: Hashemzadeh P 

PROVIDER: S-EPMC10686926 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature