Development of a novel integrated isothermal amplification system for detection of bacteria-spiked blood samples.

ABSTRACT: Bloodstream infection (BSI) caused by bacteria is highly pathogenic and lethal, and easily develops whole-body inflammatory state. Immediate identification of disease-causing bacteria can improve patient prognosis. Traditional testing methods are not only time-consuming, but such tests are limited to laboratories. Recombinase polymerase amplification combined with lateral flow dipstick (RPA-LFD) holds great promise for rapid nucleic acid detection, but the uncapping operation after amplification easily contaminates laboratories. Therefore, the establishment of a more effective integrated isothermal amplification system has become an urgent problem to be solved. In this study, we designed and fabricated a hermetically sealed integrated isothermal amplification system. Combining with this sy

SUBMITTER: Li J 

PROVIDER: S-EPMC10686969 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature