"Boundary residues" between the folded RNA recognition motif and disordered RGG domains are critical for FUS-RNA binding.

ABSTRACT: Fused in sarcoma (FUS) is an abundant RNA-binding protein, which drives phase separation of cellular condensates and plays multiple roles in RNA regulation. The RNA-binding ability of FUS protein is crucial to its cellular function. Here, our molecular simulation study on the FUS-RNA complex provides atomic resolution insights into the observations from biochemical studies and also illuminates our understanding of molecular driving forces that mediate the structure, stability, and interaction of the RNA recognition motif (RRM) and RGG domains of FUS with a stem-loop junction RNA. We observe clear cooperativity and division of labor among the ordered (RRM) and disordered domains (RGG1 and RGG2) of FUS that leads to an organized and tighter RNA binding. Irrespective of the length of RGG2, th

SUBMITTER: Balasubramanian S 

PROVIDER: S-EPMC10687056 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature