Benchmarking of bioinformatics tools for NGS-based microRNA profiling with RT-qPCR method.

ABSTRACT: MicroRNAs are vital gene expression regulators, extensively studied worldwide. The large-scale characterization of miRNAomes is possible using next-generation sequencing (NGS). This technology offers great opportunities, but these cannot be fully exploited without proper and comprehensive bioinformatics analysis. This may be achieved by the use of reliable dedicated software; however, different programs may generate divergent results, leading to additional discrepancies. Thus, the aim of this study was to compare three bioinformatic algorithms dedicated to NGS-based microRNA profiling and validate them using an alternative method, namely RT-qPCR. The comparison analysis revealed differences in the number and sets of identified miRNAs. The qPCR confirmed the expression of the investigated m

SUBMITTER: Pawlina-Tyszko K 

PROVIDER: S-EPMC10687144 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature