Adoptive cell transfer of macrophages following peripheral nerve injury in mice.

ABSTRACT: Macrophages are key innate immune cells involved in multiple biological processes, including peripheral nerve regeneration. Here, we describe a protocol for the adoptive cell transfer of bone-marrow-derived macrophages (BMDMs) following sciatic nerve crush injury (SNCI). This procedure involves isolating BMDMs from a donor mouse, potentially manipulating them ex vivo, and reintroducing them into an animal following SNCI. Preclinical studies show that BMDMs can infiltrate injured nerves and impact functional recovery, potentially providing a novel therapy for nerve injuries. For complete details on the use and execution of this protocol, please refer to Jha et al.¹.

SUBMITTER: Rawat A 

PROVIDER: S-EPMC10687290 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature