Detection of the interactions of tumour derived extracellular vesicles with immune cells is dependent on EV-labelling methods.

ABSTRACT: Cell-cell communication within the complex tumour microenvironment is critical to cancer progression. Tumor-derived extracellular vesicles (TD-EVs) are key players in this process. They can interact with immune cells and modulate their activity, either suppressing or activating the immune system. Deciphering the interactions between TD-EVs and immune cells is essential to understand immune modulation by cancer cells. Fluorescent labelling of TD-EVs is a method of choice to study such interaction. This work aims to determine the impact of EV labelling methods on the detection by imaging flow cytometry and multicolour spectral flow cytometry of EV interaction and capture by the different immune cell types within human Peripheral Blood Mononuclear Cells (PBMCs). EVs released by the triple-neg

SUBMITTER: Loconte L 

PROVIDER: S-EPMC10687762 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature