TRIM45 aggravates microglia pyroptosis via Atg5/NLRP3 axis in septic encephalopathy.

ABSTRACT:

Background

Neuroinflammation mediated by microglial pyroptosis is an important pathogenic mechanism of septic encephalopathy (SAE). It has been reported that TRIM45 is associated with tumours and inflammatory diseases. However, the role of TRIM45 in SAE and the relationship between TRIM45 and microglial pyroptosis are unknown. In this study, we found that TRIM45 played an important role in regulating microglial pyroptosis and the molecular mechanism.

Methods

SAE was induced by intraperitoneal injection of LPS in WT and AAV-shTRIM45 mice. BV2 cells were treated with LPS/ATP in vitro. Cognitive function was assessed by the Morris water maze. Nissl staining was used to evaluate histological and structural lesions. ELISA was used to dectect neuroinflammation. qPCR was used to de

SUBMITTER: Huang X 

PROVIDER: S-EPMC10688018 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature