Deciphering Solution and Gas-Phase Interactions between Peptides and Lipids by Native Mass Spectrometry.

ABSTRACT: Many biological processes depend on the interactions between proteins and lipids. Accordingly, the analysis of protein-lipid complexes has become increasingly important. Native mass spectrometry is often used to identify and characterize specific protein-lipid interactions. However, it requires the transfer of the analytes into the gas phase, where electrostatic interactions are enhanced and hydrophobic interactions do not exist. Accordingly, the question remains whether interactions that are observed in the gas phase accurately reflect interactions that are formed in solution. Here, we systematically explore noncovalent interactions between the antimicrobial peptide LL-37 and glycerophospholipids containing different headgroups or varying in fatty acyl chain length. We observe differences

SUBMITTER: Kundlacz T 

PROVIDER: S-EPMC10688224 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature