Computational analysis of protein synthesis, diffusion, and binding in compartmental biochips.

ABSTRACT: Protein complex assembly facilitates the combination of individual protein subunits into functional entities, and thus plays a crucial role in biology and biotechnology. Recently, we developed quasi-twodimensional, silicon-based compartmental biochips that are designed to study and administer the synthesis and assembly of protein complexes. At these biochips, individual protein subunits are synthesized from locally confined high-density DNA brushes and are captured on the chip surface by molecular traps. Here, we investigate single-gene versions of our quasi-twodimensional synthesis systems and introduce the trap-binding efficiency to characterize their performance. We show by mathematical and computational modeling how a finite trap density determines the dynamics of protein-trap binding

SUBMITTER: Forste S 

PROVIDER: S-EPMC10688499 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature