Pd@Pt Nanodendrites as Peroxidase Nanomimics for Enhanced Colorimetric ELISA of Cytokines with Femtomolar Sensitivity.

ABSTRACT: Colorimetric enzyme-linked immunosorbent assay (ELISA) has been widely applied as the gold-standard method for cytokine detection over decades. However, it has become a critical challenge to further improve the detection sensitivity of ELISA as limited by the catalytic activity of enzymes. Herein, we report an enhanced colorimetric ELISA for ultrasensitive detection of interleukin-6 (IL-6, as a model cytokine for demonstration) using Pd@Pt core@shell nanodendrites (Pd@Pt NDs) as peroxidase nanomimics (named "Pd@Pt ND ELISA"), pushing the sensitivity up to femtomolar level. Specifically, the Pd@Pt NDs are rationally engineered by depositing Pt atoms on Pd nanocubes (NCs) to generate rough dendrite-like Pt skins on the Pd surfaces via Volmer-Weber growth mode. They can be produced on a large

SUBMITTER: Gao Z 

PROVIDER: S-EPMC10688776 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature