Label-free SERS assay combined with multivariate spectral data analysis for lamotrigine quantification in human serum.

ABSTRACT: Considering the need for a more time and cost-effective method for lamotrigine (LTG) detection in clinics we developed a fast and robust label-free assay based on surface-enhanced Raman scattering (SERS) for LTG quantification from human serum. The optimization and application of the developed assay is presented  showing the: (i) exploration of different methods for LTG separation from human serum; (ii) implementation of a molecular adsorption step on an ordered Au nanopillar SERS substrate; (iii) adaptation of a fast scanning of the SERS substrate, performed with a custom-built compact Raman spectrometer; and (iv) development of LTG quantification methods with univariate and multivariate spectral data analysis. Our results showed, for the first time, the SERS-based characterization of LTG

SUBMITTER: Badillo-Ramirez I 

PROVIDER: S-EPMC10689517 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature