Production of perillic acid from orange essential oil by Yarrowia lipolytica using a top-aerated bioreactor.

ABSTRACT: R-(+)-Perillic acid, a promising anticancer and immunomodulatory agent, is the major product from the biotransformation of R-(+)-limonene-rich orange essential oil by the yeast Yarrowia lipolytica. Due to the abundance and low cost of orange essential oil, which is a byproduct of the citrus industry, we attempted to improve the biotransformation process by optimizing yeast cell mass production. Then, the whole process was transposed and adapted to a 2-L instrumented bioreactor. Cell mass production was optimized in shaker flasks using a statistical experimental design. The optimized medium (g·L^-1: 22.9 glucose, 7.7 peptone, 4.1 yeast extract and 1.0 malt extract) resulted in a 13.0 g·L^-1 final cell concentration and 0.18 g cell·L^-1·h^-1 productivi

SUBMITTER: Knopp FM 

PROVIDER: S-EPMC10689591 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature