An arginine-rich nuclear localization signal (ArgiNLS) strategy for streamlined image segmentation of single-cells.

ABSTRACT: High-throughput volumetric fluorescent microscopy pipelines can spatially integrate whole-brain structure and function at the foundational level of single-cells. However, conventional fluorescent protein (FP) modifications used to discriminate single-cells possess limited efficacy or are detrimental to cellular health. Here, we introduce a synthetic and non-deleterious nuclear localization signal (NLS) tag strategy, called 'Arginine-rich NLS' (ArgiNLS), that optimizes genetic labeling and downstream image segmentation of single-cells by restricting FP localization near-exclusively in the nucleus through a poly-arginine mechanism. A single N-terminal ArgiNLS tag provides modular nuclear restriction consistently across spectrally separate FP variants. ArgiNLS performance in vivo displays fun

SUBMITTER: Szelenyi ER 

PROVIDER: S-EPMC10690249 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature