METTL3 alters capping enzyme expression and its activity on ribosomal proteins.

ABSTRACT: The 5' cap, catalyzed by RNA guanylyltransferase and 5'-phosphatase (RNGTT), is a vital mRNA modification for the functionality of mRNAs. mRNA capping occurs in the nucleus for the maturation of the functional mRNA and in the cytoplasm for fine-tuning gene expression. Given the fundamental importance of RNGTT in mRNA maturation and expression there is a need to further investigate the regulation of RNGTT. N6-methyladenosine (m⁶A) is one of the most abundant RNA modifications involved in the regulation of protein translation, mRNA stability, splicing, and export. We sought to investigate whether m⁶A could regulate the expression and activity of RNGTT. A motif for the m⁶A writer methyltransferase 3 (METTL3) in the 3'UTR of RNGTT mRNA was identified. Knockdown

SUBMITTER: Del Valle-Morales D 

PROVIDER: S-EPMC10690260 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature