Epigenetic regulation limits competence of pluripotent stem cell-derived oocytes.

ABSTRACT: Recent studies have reported the differentiation of pluripotent cells into oocytes in vitro. However, the developmental competence of in vitro-generated oocytes remains low. Here, we perform a comprehensive comparison of mouse germ cell development in vitro over all culture steps versus in vivo with the goal to understand mechanisms underlying poor oocyte quality. We show that the in vitro differentiation of primordial germ cells to growing oocytes and subsequent follicle growth is critical for competence for preimplantation development. Systematic transcriptome analysis of single oocytes that were subjected to different culture steps identifies genes that are normally upregulated during oocyte growth to be susceptible for misregulation during in vitro oogenesis. Many misregulated genes ar

SUBMITTER: Aizawa E 

PROVIDER: S-EPMC10690455 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature