Fast HPLC-based affinity method to determine capsid titer and full/empty ratio of adeno-associated viral vectors.

ABSTRACT: Recombinant adeno-associated viruses (rAAVs) are promising gene delivery vectors in the emerging field of in vivo gene therapies. To ensure their consistent quality during manufacturing and process development, multiple analytical techniques have been proposed for the characterization and quantification of rAAV capsids. Despite their indisputable capabilities for performing this task, current analytical methods are rather time-consuming, material intensive, complicated, and costly, restricting their suitability for process development in which time and sample throughput are severe constraints. To eliminate this bottleneck, we introduce here an affinity-based high-performance liquid chromatography method that allows the determination of the capsid titer and the full/empty ratio of rA

SUBMITTER: Heckel J 

PROVIDER: S-EPMC10690635 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature