Cut site preference allows influenza A virus PA-X to discriminate between host and viral mRNAs.

ABSTRACT: Many viruses block host gene expression to take over the infected cell. This process, termed host shutoff, is thought to promote viral replication by preventing antiviral responses and redirecting cellular resources to viral processes. Several viruses from divergent families accomplish host shutoff through RNA degradation by endoribonucleases. However, viruses also need to ensure expression of their own genes. The influenza A virus endoribonuclease PA-X solves this problem by sparing viral mRNAs and some host RNAs necessary for viral replication. To understand how PA-X distinguishes between RNAs, we characterized PA-X cut sites transcriptome-wide using 5' rapid amplification of complementary DNA ends coupled to high-throughput sequencing. This analysis, along with RNA structure predictions

SUBMITTER: Gaucherand L 

PROVIDER: S-EPMC10690756 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature