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Multiplexed Digital Characterization of Misfolded Protein Oligomers via Solid-State Nanopores.


ABSTRACT: Misfolded protein oligomers are of central importance in both the diagnosis and treatment of Alzheimer's and Parkinson's diseases. However, accurate high-throughput methods to detect and quantify oligomer populations are still needed. We present here a single-molecule approach for the detection and quantification of oligomeric species. The approach is based on the use of solid-state nanopores and multiplexed DNA barcoding to identify and characterize oligomers from multiple samples. We study α-synuclein oligomers in the presence of several small-molecule inhibitors of α-synuclein aggregation as an illustration of the potential applicability of this method to the development of diagnostic and therapeutic methods for Parkinson's disease.

SUBMITTER: Sandler SE 

PROVIDER: S-EPMC10690769 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature

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Multiplexed Digital Characterization of Misfolded Protein Oligomers via Solid-State Nanopores.

Sandler Sarah E SE   Horne Robert I RI   Rocchetti Sara S   Novak Robert R   Hsu Nai-Shu NS   Castellana Cruz Marta M   Faidon Brotzakis Z Z   Gregory Rebecca C RC   Chia Sean S   Bernardes Gonçalo J L GJL   Keyser Ulrich F UF   Vendruscolo Michele M  

Journal of the American Chemical Society 20231116 47


Misfolded protein oligomers are of central importance in both the diagnosis and treatment of Alzheimer's and Parkinson's diseases. However, accurate high-throughput methods to detect and quantify oligomer populations are still needed. We present here a single-molecule approach for the detection and quantification of oligomeric species. The approach is based on the use of solid-state nanopores and multiplexed DNA barcoding to identify and characterize oligomers from multiple samples. We study α-s  ...[more]

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