Alfalfa vein mottling virus, a novel potyvirid infecting Medicago sativa L.

ABSTRACT:

Background

We have recently identified a novel virus detected in alfalfa seed material. The virus was tentatively named alfalfa-associated potyvirus 1, as its genomic fragments bore similarities with potyvirids. In this study, we continued investigating this novel species, expanding information on its genomic features and biological characteristics.

Methods

This research used a wide range of methodology to achieve end results: high throughput sequencing, bioinformatics tools, reverse transcription-polymerase chain reactions, differential diagnostics using indicator plants, virus purification, transmission electron microscopy, and others.

Results

In this study, we obtained a complete genome sequence of the virus and classified it as a tentative species in the new genus

SUBMITTER: Nemchinov LG 

PROVIDER: S-EPMC10690988 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature