Dataset Information

Soybean reduced internode 1 determines internode length and improves grain yield at dense planting.

ABSTRACT: Major cereal crops have benefitted from Green Revolution traits such as shorter and more compact plants that permit high-density planting, but soybean has remained relatively overlooked. To balance ideal soybean yield with plant height under dense planting, shortening of internodes without reducing the number of nodes and pods is desired. Here, we characterized a short-internode soybean mutant, reduced internode 1 (rin1). Partial loss of SUPPRESSOR OF PHYA 105 3a (SPA3a) underlies rin1. RIN1 physically interacts with two homologs of ELONGATED HYPOCOTYL 5 (HY5), STF1 and STF2, to promote their degradation. RIN1 regulates gibberellin metabolism to control internode development through a STF1/STF2-GA2ox7 regulatory module. In field trials, rin1 significantly enhances grain yield under high-density planting conditions comparing to its wild type of elite cultivar. rin1 mutants therefore could serve as valuable resources for improving grain yield under high-density cultivation and in soybean-maize intercropping systems.

SUBMITTER: Li S

PROVIDER: S-EPMC10692089 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature

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Soybean reduced internode 1 determines internode length and improves grain yield at dense planting.

Li Shichen S Sun Zhihui Z Sang Qing Q Qin Chao C Kong Lingping L Huang Xin X Liu Huan H Su Tong T Li Haiyang H He Milan M Fang Chao C Wang Lingshuang L Liu Shuangrong S Liu Bin B Liu Baohui B Fu Xiangdong X Kong Fanjiang F Lu Sijia S

Nature communications 20231201 1

Major cereal crops have benefitted from Green Revolution traits such as shorter and more compact plants that permit high-density planting, but soybean has remained relatively overlooked. To balance ideal soybean yield with plant height under dense planting, shortening of internodes without reducing the number of nodes and pods is desired. Here, we characterized a short-internode soybean mutant, reduced internode 1 (rin1). Partial loss of SUPPRESSOR OF PHYA 105 3a (SPA3a) underlies rin1. RIN1 phy ...[more]

PMID: 38040709

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Project description:A major challenge for utilizing cannabis for modern medicine is the spatial variability of cannabinoids in the plant, which entail differences in medical potency. Since secondary metabolism is affected by environmental conditions, a key trigger for the variability in secondary metabolites throughout the plant is variation in local micro-climates. We have, therefore, hypothesized that plant density, which is well-known to alter micro-climate in the canopy, affects spatial standardization, and concentrations of cannabinoids in cannabis plants. Canopy density is affected by shoot architecture and by plant spacing, and we have therefore evaluated the interplay between plant architecture and plant density on the standardization of the cannabinoid profile in the plant. Four plant architecture modulation treatments were employed on a drug-type medicinal cannabis cultivar, under a density of 1 or 2 plants/m2. The plants were cultivated in a naturally lit greenhouse with photoperiodic light supplementation. Analysis of cannabinoid concentrations at five locations throughout the plant was used to evaluate treatment effects on chemical uniformity. The results revealed an effect of plant density on cannabinoid standardization, as well as an interaction between plant density and plant architecture on the standardization of cannabinoids, thus supporting the hypothesis. Increasing planting density from 1 to 2 plants/m2 reduced inflorescence yield/plant, but increased yield quantity per area by 28-44% in most plant architecture treatments. The chemical response to plant density and architecture modulation was cannabinoid-specific. Concentrations of cannabinoids in axillary inflorescences from the bottom of the plants were up to 90% lower than in the apical inflorescence at the top of the plant, considerably reducing plant uniformity. Concentrations of all detected cannabinoids in these inflorescences were lower at the higher density plants; however, cannabinoid yield per cultivation area was not affected by neither architecture nor density treatments. Cannabigerolic acid (CBGA) was the cannabinoid least affected by spatial location in the plant. The morpho-physiological response of the plants to high density involved enhanced leaf drying at the bottom of the plants, increased plant elongation, and reduced cannabinoid concentrations, suggesting an involvement of chronic light deprivation at the bottom of the plants. Therefore, most importantly, under high density growth, architectural modulating treatments that facilitate increased light penetration to the bottom of the plant such as "Defoliation", or that eliminated inflorescences development at the bottom of the plant such as removal of branches from the lower parts of the plant, increased chemical standardization. This study revealed the importance of plant density and architecture for chemical quality and standardization in drug-type medical cannabis.

Project description:BackgroundSoybean (Glycine max) is an economically important oil and protein crop. Plant height is a key trait that significantly impacts the yield of soybean; however, research on the molecular mechanisms associated with soybean plant height is lacking. The CRISPR (clustered regularly interspaced short palindromic repeat)/Cas9 (CRISPR-associated system 9) system is a recently developed technology for gene editing that has been utilized to edit the genomes of crop plants.ResultsHere, we designed four gRNAs to mutate four LATE ELONGATED HYPOCOTYL (LHY) genes in soybean. In order to test whether the gRNAs could perform properly in transgenic soybean plants, we first tested the CRISPR construct in transgenic soybean hairy roots using Agrobacterium rhizogenes strain K599. Once confirmed, we performed stable soybean transformation and obtained 19 independent transgenic soybean plants. Subsequently, we obtained one T1 transgene-free homozygous quadruple mutant of GmLHY by self-crossing. The phenotypes of the T2-generation transgene-free quadruple mutant plants were observed, and the results showed that the quadruple mutant of GmLHY displayed reduced plant height and shortened internodes. The levels of endogenous gibberellic acid (GA3) in Gmlhy1a1b2a2b was lower than in the wild type (WT), and the shortened internode phenotype could be rescued by treatment with exogenous GA3. In addition, the relative expression levels of GA metabolic pathway genes in the quadruple mutant of GmLHY were significantly decreased in comparison to the WT. These results suggest that GmLHY encodes an MYB transcription factor that affects plant height through mediating the GA pathway in soybean. We also developed genetic markers for identifying mutants for application in breeding studies.ConclusionsOur results indicate that CRISPR/Cas9-mediated targeted mutagenesis of four GmLHY genes reduces soybean plant height and shortens internodes from 20 to 35 days after emergence (DAE). These findings provide insight into the mechanisms underlying plant height regulatory networks in soybean.

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Soybean reduced internode 1 determines internode length and improves grain yield at dense planting.

Publications

Soybean reduced internode 1 determines internode length and improves grain yield at dense planting.

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