A rapid and stable spontaneous reprogramming system of Spermatogonial stem cells to Pluripotent State.

ABSTRACT:

Background

The scarcity of pluripotent stem cells poses a major challenge to the clinical application, given ethical and biosafety considerations. While germline stem cells commit to gamete differentiation throughout life, studies demonstrated the spontaneous acquisition of pluripotency by spermatogonial stem cells (SSCs) from neonatal testes at a low frequency (1 in 1.5 × 10⁷). Notably, this process occurs without exogenous oncogenes or chemical supplementation. However, while knockout of the p53 gene accelerates the transformation of SSCs, it also increases risk and hampers their clinical use.

Results

We report a transformation system that efficiently and stably convert SSCs into pluripotent stem cells around 10 passages with the morphology similar to that of ep

SUBMITTER: Wei R 

PROVIDER: S-EPMC10693117 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature