Transcription factor ETV4 promotes the development of hepatocellular carcinoma by driving hepatic TNF-α signaling.

ABSTRACT:

Background

Hepatic inflammation is the major risk factor of hepatocellular carcinoma (HCC). However, the underlying mechanism by which hepatic inflammation progresses to HCC is poorly understood. This study was designed to investigate the role of ETS translocation variant 4 (ETV4) in linking hepatic inflammation to HCC.

Methods

Quantitative real-time PCR and immunoblotting were used to detect the expression of ETV4 in HCC tissues and cell lines. RNA sequencing and luciferase reporter assays were performed to identify the target genes of ETV4. Hepatocyte-specific ETV4-knockout (ETV4^{fl/fl, alb-cre} ) and transgenic (ETV4^Hep-TG ) mice and diethylnitrosamine-carbon tetrachloride (DEN-CCL₄ ) treatment experiments were applied to investigate the fun

SUBMITTER: Qi D 

PROVIDER: S-EPMC10693303 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature