Novel WYL domain-containing transcriptional activator acts in response to genotoxic stress in rapidly growing mycobacteria.

ABSTRACT: The WYL domain is a nucleotide-sensing module that controls the activity of transcription factors involved in the regulation of DNA damage response and phage defense mechanisms in bacteria. In this study, we investigated a WYL domain-containing transcription factor in Mycobacterium smegmatis that we termed stress-involved WYL domain-containing regulator (SiwR). We found that SiwR controls adjacent genes that belong to the DinB/YfiT-like putative metalloenzymes superfamily by upregulating their expression in response to various genotoxic stress conditions, including upon exposure to H₂O₂ or the natural antibiotic zeocin. We show that SiwR binds different forms of single-stranded DNA (ssDNA) with high affinity, primarily through its characteristic WYL domain. In combina

SUBMITTER: Keller LML 

PROVIDER: S-EPMC10693628 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature