Development and pre-clinical evaluation of a Zika virus diagnostic for low resource settings.

ABSTRACT:

Introduction

Zika virus (ZIKV) is a re-emerging flavivirus that poses a significant public health threat. ZIKV exhibits a wide array of non-vector borne human transmission routes, such as sexual transmission, transplacental transmission and blood transfusion. Detection and surveillance of ZIKV is considered paramount in prevention of major outbreaks. With the majority of cases reported in low-resource locations, simple, low-cost detection methods are considered highly desirable.

Materials and methods

Here we have developed a sensitive and specific ZIKV diagnostic using reverse transcription recombinase-aided amplification (RT-RAA) coupled with lateral flow detection (LFD) targeting a highly conserved region of the ZIKV NS1 gene.

Results

We show our rapid, isothermal-Z

SUBMITTER: Balea R 

PROVIDER: S-EPMC10694267 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature