CAMP competitively inhibits periplasmic phosphatases to coordinate nutritional growth with competence of Haemophilus influenzae.

ABSTRACT: Most naturally competent bacteria tightly regulate the window of the competent state to maximize their ecological fitness under specific conditions. Development of competence by Haemophilus influenzae strain Rd KW20 is stimulated by cAMP and inhibited by purine nucleotides, respectively. In contrast, cAMP inhibits cell growth, but nucleotides are important for KW20 growth. However, the mechanisms underlying the abovementioned reciprocal effects are unclear. Here, we first identified a periplasmic acid phosphatase AphA_Ec of Escherichia coli as a new cAMP-binding protein. We show cAMP competitively inhibits the phosphatase activities of AphA_Ec and its homolog protein AphA_Hi in the KW20 strain. Furthermore, we found cAMP inhibits two other periplasmic nonspeci

SUBMITTER: Kronborg K 

PROVIDER: S-EPMC10694654 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature