Identification of the murine AAVrh32.33 capsid-specific CD8+ T cell epitopes.

ABSTRACT:

Background

Adeno-associated virus (AAV) is an ideal gene therapy vector and is non-immunogenic in many small animal models. The stable gene expression commonly seen in murine models does not necessarily translate to nonhuman primates and higher-order species, highlighting the need for a better understanding of immune activation to these vectors. One capsid variant, AAVrh32.33, demonstrates a unique phenotype in murine muscle, reminiscent of what is often seen in higher-order species. AAVrh32.33 generates a strong CD8+ T-cell response to both capsid and encoded transgene antigens in a manner independent of transgene product or major histocompatability complex haplotype, making it an ideal candidate for studying immune activation to AAV in the mouse.

Methods

To map the H-2b an

SUBMITTER: Mays LE 

PROVIDER: S-EPMC10694856 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature