Identification of postnatal development dependent genes and proteins in porcine epididymis.

ABSTRACT:

Background

The epididymis is a highly regionalized tubular organ possesses vectorial functions of sperm concentration, maturation, transport, and storage. The epididymis-expressed genes and proteins are characterized by regional and developmental dependent pattern. However, a systematic and comprehensive insight into the postnatal development dependent changes in gene and protein expressions of porcine epididymis is still lacking. Here, the RNA and protein of epididymis of Duroc pigs at different postnatal development stages were extracted by using commercial RNeasy Midi kit and extraction buffer (7 M Urea, 2 M thiourea, 3% CHAPS, and 1 mM PMSF) combined with sonication, respectively, which were further subjected to transcriptomic and proteomic profiling.

Results

Transcripto

SUBMITTER: Fang S 

PROVIDER: S-EPMC10694963 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature