Quantification of functional recovery in a larval zebrafish model of spinal cord injury.

ABSTRACT: Human spinal cord injury (SCI) is characterized by permanent loss of damaged axons, resulting in chronic disability. In contrast, zebrafish can regenerate axonal projections following central nervous system injury and re-establish synaptic contacts with distant targets; elucidation of the underlying molecular events is an important goal with translational potential for improving outcomes in SCI patients. We generated transgenic zebrafish with GFP-labeled axons and transected their spinal cords at 10 days post-fertilization. Intravital confocal microscopy revealed robust axonal regeneration following the procedure, with abundant axons bridging the transection site by 48 h post-injury. In order to analyze neurological function in this model, we developed and validated new open-source softwar

SUBMITTER: Hossainian D 

PROVIDER: S-EPMC10695274 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature