Identification of cis-regulatory sequences in the human angiotensinogen gene by transgene coplacement and site-specific recombination.

ABSTRACT: The function of putative regulatory sequences identified in cell transfection experiments can be elucidated only through in vivo experimentation. However, studies of gene regulation in transgenic mice (TgM) are often compromised by the position effects, in which independent transgene insertions differ in expression depending on their location in the genome. In order to overcome such a dilemma, a method called transgene coplacement has been developed in Drosophila melanogaster. In this method, any two sequences can be positioned at exactly the same genomic site by making use of Cre/loxP recombination. Here we applied this method to mouse genetics to characterize the function of direct repeat (DR) sequences in the promoter of the human angiotensinogen (hAGT) gene, the precursor of the vasoac

SUBMITTER: Shimizu T 

PROVIDER: S-EPMC1069595 | biostudies-literature | 2005 Apr

REPOSITORIES: biostudies-literature