REC domain stabilizes the active heptamer of σ⁵⁴-dependent transcription factor, FleR from Pseudomonas aeruginosa.

ABSTRACT: Motility in Pseudomonas aeruginosa is mediated through a single, polar flagellum, which is essential for virulence, colonization, and biofilm formation. FleSR, a two-component system (TCS), serves as a critical checkpoint in flagellar assembly. FleR is a σ⁵⁴-dependent response regulator that undergoes phosphorylation via cognate sensor kinase FleS for the assembly of the functionally active form. The active form remodels the σ⁵⁴-RNAP complex to initiate transcription. Small-angle X-ray scattering, crystallography, and negative staining electron microscopy reconstructions of FleR revealed that it exists predominantly as a dimer in the inactive form with low ATPase activity and assembles into heptamers upon phosphorylation with amplified ATPase activity. We estab

SUBMITTER: Sahoo PK 

PROVIDER: S-EPMC10696123 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature