Application of Liquid-Liquid Extraction for N-terminal Myristoylation Proteomics.

ABSTRACT: Proteins can be modified by lipids in various ways, for example, by myristoylation, palmitoylation, farnesylation, and geranylgeranylation-these processes are collectively referred to as lipidation. Current chemical proteomics using alkyne lipids has enabled the identification of lipidated protein candidates but does not identify endogenous lipidation sites and is not readily applicable to in vivo systems. Here, we introduce a proteomic methodology for global analysis of endogenous protein N-terminal myristoylation sites that combines liquid-liquid extraction of hydrophobic lipidated peptides with liquid chromatography-tandem mass spectrometry using a gradient program of acetonitrile in the high concentration range. We applied this method to explore myristoylation sites in HeLa cells and i

SUBMITTER: Tsumagari K 

PROVIDER: S-EPMC10696250 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature