SBioSITe enables sensitive identification of the cell surface proteome through direct enrichment of biotinylated peptides.

ABSTRACT:

Background

Cell surface proteins perform critical functions related to immune response, signal transduction, cell-cell interactions, and cell migration. Expression of specific cell surface proteins can determine cell-type identity, and can be altered in diseases including infections, cancer and genetic disorders. Identification of the cell surface proteome remains a challenge despite several enrichment methods exploiting their biochemical and biophysical properties.

Methods

Here, we report a novel method for enrichment of proteins localized to cell surface. We developed this new approach designated surface Biotinylation Site Identification Technology (sBioSITe) by adapting our previously published method for direct identification of biotinylated peptides. In this strategy, t

SUBMITTER: Garapati K 

PROVIDER: S-EPMC10696767 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature