Design of stimulus-responsive two-state hinge proteins.
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ABSTRACT:
SUBMITTER: Praetorius F
PROVIDER: S-EPMC10697137 | biostudies-literature | 2023 Aug
REPOSITORIES: biostudies-literature
Publications
Design of stimulus-responsive two-state hinge proteins.
Praetorius Florian F Leung Philip J Y PJY Tessmer Maxx H MH Broerman Adam A Demakis Cullen C Dishman Acacia F AF Pillai Arvind A Idris Abbas A Juergens David D Dauparas Justas J Li Xinting X Levine Paul M PM Lamb Mila M Ballard Ryanne K RK Gerben Stacey R SR Nguyen Hannah H Kang Alex A Sankaran Banumathi B Bera Asim K AK Volkman Brian F BF Nivala Jeff J Stoll Stefan S Baker David D
Science (New York, N.Y.) 20230817 6659
In nature, proteins that switch between two conformations in response to environmental stimuli structurally transduce biochemical information in a manner analogous to how transistors control information flow in computing devices. Designing proteins with two distinct but fully structured conformations is a challenge for protein design as it requires sculpting an energy landscape with two distinct minima. Here we describe the design of "hinge" proteins that populate one designed state in the absen ...[more]
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