Spatiotemporal protein dynamics during early organogenesis in mouse conceptuses treated with valproic acid.

ABSTRACT: Valproic acid (VPA) is an anti-epileptic medication that increases the risk of neural tube defect (NTD) outcomes in infants exposed during gestation. Previous studies into VPA's mechanism of action have focused on alterations in gene expression and metabolism but have failed to consider how exposure changes the abundance of critical developmental proteins over time. This study evaluates the effects of VPA on protein abundance in the developmentally distinct tissues of the mouse visceral yolk sac (VYS) and embryo proper (EMB) using mouse whole embryo culture. Embryos were exposed to 600 μM VPA at 2 h intervals over 10 h during early organogenesis with the aim of identifying protein pathways relevant to VPA's mechanism of action in failed NTC. Protein abundance was measured through tandem ma

SUBMITTER: Lapehn S 

PROVIDER: S-EPMC10697214 | biostudies-literature | 2023 Sep-Oct

REPOSITORIES: biostudies-literature