Biofilm dysbiosis and caries activity: a surface or an individual issue?

ABSTRACT:

Objective

This study aimed to analyze the functional profile of supragingival biofilm from sound (CAs), active (CAa), and inactive (CAi) enamel caries lesions from caries-active individuals to provide insights into the diversity of biological processes regarding biofilm dysbiosis.

Methodology

A metatranscriptome analysis was performed in biofilm samples collected from five caries-active individuals. Total RNA was extracted, and the microbial cDNAs were obtained and sequenced (Illumina HiSeq3000). Trimmed data were submitted to the SqueezeMeta pipeline in the co-assembly mode for functional analysis and further differential gene expression analysis (DESeq2).

Results

Bioinformatics analysis of mRNAs revealed a similar functional profile related to all analyzed condition

SUBMITTER: Ev LD 

PROVIDER: S-EPMC10697669 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature