Methionine-producing tumor micro(be) environment fuels growth of solid tumors.

ABSTRACT:

Background

Recent studies have uncovered the near-ubiquitous presence of microbes in solid tumors of diverse origins. Previous literature has shown the impact of specific bacterial species on the progression of cancer. We propose that local microbial dysbiosis enables certain cancer phenotypes through provisioning of essential metabolites directly to tumor cells.

Methods

16S rDNA sequencing of 75 patient lung samples revealed the lung tumor microbiome specifically enriched for bacteria capable of producing methionine. Wild-type (WT) and methionine auxotrophic (metA mutant) E. coli cells were used to condition cell culture media and the proliferation of lung adenocarcinoma (LUAD) cells were measured using SYTO60 staining. Further, colony forming assay, Annexin V Staining, Brd

SUBMITTER: Vega AA 

PROVIDER: S-EPMC10697899 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature