National genomic surveillance integrating standardized quantitative susceptibility testing clarifies antimicrobial resistance in Enterobacterales.

ABSTRACT: Antimicrobial resistance is a global health concern; Enterobacterales resistant to third-generation cephalosporins (3GCs) and carbapenems are of the highest priority. Here, we conducted genome sequencing and standardized quantitative antimicrobial susceptibility testing of 4,195 isolates of Escherichia coli and Klebsiella pneumoniae resistant to 3GCs and Enterobacterales with reduced meropenem susceptibility collected across Japan. Our analyses provided a complete classification of 3GC resistance mechanisms. Analyses with complete reference plasmids revealed that among the bla_CTX-M extended-spectrum β-lactamase genes, bla_CTX-M-8 was typically encoded in highly similar plasmids. The two major AmpC β-lactamase genes were bla_CMY-2 and bla_DHA-1. Long

SUBMITTER: Kayama S 

PROVIDER: S-EPMC10698200 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature