Cholangiocarcinoma-on-a-chip: A human 3D platform for personalised medicine.

ABSTRACT:

Background & aims

Cholangiocarcinoma (CCA) is a primary liver tumour characterised by a poor prognosis and limited therapeutic options. Available 3D human CCA models fail to faithfully recapitulate the tumour niche. We aimed to develop an innovative patient-specific CCA-on-chip platform.

Methods

A CCA tumour microenvironment was recapitulated on a microfluidic three-channel chip using primary CCA cells, cancer-associated fibroblasts (CAFs), endothelial cells, and T cells isolated from CCA specimens (n = 6). CAF and CCA cells were co-cultured in the central channel, flanked by endothelial cells in one lateral channel, recreating a tubular structure. An extensive characterisation of this platform was carried out to investigate its diffusion ability, hydrogel properties, and ch

SUBMITTER: Polidoro MA 

PROVIDER: S-EPMC10698278 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature