Advancing animal tuberculosis surveillance using culture-independent long-read whole-genome sequencing.

ABSTRACT: Animal tuberculosis is a significant infectious disease affecting both livestock and wildlife populations worldwide. Effective disease surveillance and characterization of Mycobacterium bovis (M. bovis) strains are essential for understanding transmission dynamics and implementing control measures. Currently, sequencing of genomic information has relied on culture-based methods, which are time-consuming, resource-demanding, and concerning in terms of biosafety. This study explores the use of culture-independent long-read whole-genome sequencing (WGS) for a better understanding of M. bovis epidemiology in African buffaloes (Syncerus caffer). By comparing two sequencing approaches, we evaluated the efficacy of Illumina WGS performed on culture extracts and culture-indepe

SUBMITTER: Ghielmetti G 

PROVIDER: S-EPMC10699144 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature