Raman-dielectrophoresis goes viral: towards a rapid and label-free platform for plant virus characterization.

ABSTRACT: An innovative spectroscopic method that allows to chemically and structurally characterize viruses directly in suspension within few minutes was developed. A library of five different plant viruses was obtained combining dielectrophoresis (DEP), performed with a device specifically designed to capture and agglomerate virus particles, and Raman spectroscopy to provide a chemical fingerprint of virions. The tested viruses, purified from infected plants, were chosen for their economic impact on horticultural crops and for their different morphological and structural features. Using the Raman-DEP device, specific profiles for each virus were successfully obtained, relying on chemical differences occurring even with genetically similar viruses belonging to the same taxonomic species and morphol

SUBMITTER: Sacco A 

PROVIDER: S-EPMC10699305 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature