Cooperative Response to Endocardial Notch Reveals Interaction With Hippo Pathway.

ABSTRACT:

Background

The endocardium is a crucial signaling center for cardiac valve development and maturation. Genetic analysis has identified several human endocardial genes whose inactivation leads to bicuspid aortic valve formation and calcific aortic valve disease, but knowledge is very limited about the role played in valve development and disease by noncoding endocardial regulatory regions and upstream factors.

Methods

We manipulated Notch signaling in mouse embryonic endocardial cells by short-term and long-term coculture with OP9 stromal cells expressing Notch ligands and inhibition of Notch activity. We examined the transcriptional profile and chromatin accessibility landscape for each condition, integrated transcriptomic, transcription factor occupancy, chromatin accessibi

SUBMITTER: Luna-Zurita L 

PROVIDER: S-EPMC10699509 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature