Development and evaluation of neutralizing antibodies for cross-protection against West Nile virus and Japanese encephalitis virus.

ABSTRACT:

Background

West Nile virus is a severe zoonotic pathogen that can cause severe central nervous system symptoms in humans and horses, and is fatal for birds, chickens and other poultry. With no specific drugs or vaccines available, antibody-based therapy is a promising treatment. This study aims to develop neutralizing antibodies against West Nile virus and assess their cross-protective potential against Japanese encephalitis virus.

Methods

Monoclonal antibodies against WNV and JEV were isolated by hybridoma technology. The therapeutic efficacy of these antibodies was evaluated using a mouse model, and a humanized version of the monoclonal antibody was generated for potential human application.

Results

In this study, we generated eight monoclonal antibodies that exhibi

SUBMITTER: Yang MJ 

PROVIDER: S-EPMC10699678 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature