Direct purification and immobilization of his-tagged enzymes using unmodified nickel ferrite NiFe₂O₄ magnetic nanoparticles.

ABSTRACT: Purification of valuable engineered proteins and enzymes can be laborious, costly, and generating large amount of chemical waste. Whilst enzyme immobilization can enhance recycling and reuse of enzymes, conventional methods for immobilizing engineered enzymes from purified samples are also inefficient with multiple-step protocols, regarding both the carrier preparation and enzyme binding. Nickel ferrite magnetic nanoparticles (NiFe₂O₄ MNPs) offer distinct advantages in both purification and immobilization of enzymes. In this work, we demonstrate the preparation of NiFe₂O₄ MNPs via a one-step solvothermal synthesis and their use in direct enzyme binding from cell lysates. These NiFe₂O₄ MNPs have showed an average diameter of

SUBMITTER: Lau ECHT 

PROVIDER: S-EPMC10700653 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature