Formation of Giant Unilamellar Vesicles Assisted by Fluorinated Nanoparticles.

ABSTRACT: In the quest to produce artificial cells, one key challenge that remains to be solved is the recreation of a complex cellular membrane. Among the existing models, giant unilamellar vesicles (GUVs) are particularly interesting due to their intrinsic compartmentalisation ability and their resemblance in size and shape to eukaryotic cells. Many techniques have been developed to produce GUVs all having inherent advantages and disadvantages. Here, the authors show that fluorinated silica nanoparticles (FNPs) used to form Pickering emulsions in a fluorinated oil can destabilise lipid nanosystems to template the formation of GUVs. This technique enables GUV production across a broad spectrum of buffer conditions, while preventing the leakage of the encapsulated components into the oil phase. Furt

SUBMITTER: Waeterschoot J 

PROVIDER: S-EPMC10700689 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature