Cold-induced suspension and resetting of Ca²⁺ and transcriptional rhythms in the suprachiasmatic nucleus neurons.

ABSTRACT: Does the circadian clock keep running under such hypothermic states as daily torpor and hibernation? This fundamental question has been a research subject for decades but has remained unsettled. We addressed this subject by monitoring the circadian rhythm of clock gene transcription and intracellular Ca²⁺ in the neurons of the suprachiasmatic nucleus (SCN), master circadian clock, in vitro under a cold environment. We discovered that the transcriptional and Ca²⁺ rhythms are maintained at 22°C-28°C, but suspended at 15°C, accompanied by a large Ca²⁺ increase. Rewarming instantly resets the Ca²⁺ rhythms, while transcriptional rhythms reach a stable phase after the transient state and recover their phase relationship with the Ca²⁺

SUBMITTER: Enoki R 

PROVIDER: S-EPMC10700853 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature