Enhancing generation efficiency of liver organoids in a collagen scaffold using human chemically derived hepatic progenitors.

ABSTRACT:

Backgrounds/aims

Liver organoids have emerged as a powerful tool for studying liver biology and disease and for developing new therapies and regenerative medicine approaches. For organoid culture, Matrigel, a type of extracellular matrix, is the most commonly used material. However, Matrigel cannot be used for clinical applications due to the presence of unknown proteins that can cause immune rejection, batch-to-batch variability, and angiogenesis.

Methods

To obtain human primary hepatocytes (hPHs), we performed 2 steps collagenase liver perfusion protocol. We treated three small molecules cocktails (A83-01, CHIR99021, and HGF) for reprogramming the hPHs into human chemically derived hepatic progenitors (hCdHs) and used hCdHs to generate liver organoids.

Results

In th

SUBMITTER: Kim M 

PROVIDER: S-EPMC10700938 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature